Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting NAT10 with Remodelin reshapes the pro-fibrotic microenvironment in renal fibrogenesis via inhibiting exosome oversecretion from tubular cells

doi: 10.1016/j.apsb.2026.05.020

Figure Lengend Snippet: Targeting acetyltransferase NAT10 with Remodelin breaks the TEC-fibroblast pro-fibrotic chain (A) The graph indicating the selection strategy of screen based on in vitro model of TEC-fibroblast communication with epigenetic inhibitor compounds. (B) The heatmap of viability of NRK-49F cells incubated with CM from HK-2 cells with indicated treatments respectively (left panel). The dots indicated the average fold of viability of NRK-49F cells incubated with CM from HK-2 cells treated with TGF- β and epigenetic inhibitors, in relative to the NRK-49F cells incubated with CM from HK-2 cells treated with TGF- β and DMSO (right panel). The red dots indicated the selected inhibitors for further screen. (C) The heatmap of viability of NRK-49F cells incubated with CM from HK-2 cells with indicated treatments, respectively. (D) The heatmap (left) and table (right) demonstrating the details of 13 effective epigenetic inhibitors. (E) The immunoblot analysis of NAT10 in kidneys from sham mice ( n = 5) and UUO mice ( n = 5). (F) The dot blot analysis of ac4C in kidneys from sham mice ( n = 2) and UUO mice ( n = 2). (G) The immunohistochemistry staining of NAT10 in kidneys from sham mice and UUO mice. (H) The immunofluorescence staining of NAT10 in kidneys from sham mice and UUO mice. LTL was used as marker of proximal tubular cells. (I) The immunoblot analysis of NAT10 in kidneys from CKD patients ( n = 18) and non-CKD control ( n = 5). (J) The dot blot analysis of ac4C in kidneys from CKD patients ( n = 18) and non-CKD control ( n = 5). Data are presented as mean ± SD. Student's t test was used for comparison between two groups and one-way ANOVA was used for comparison between multiple groups; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: HK-2 cells were seeded in 96-well plates at the density of 10,000 cells per well, and then treated with TGF- β (5 ng/mL) in the presence of each inhibitor (1 or 5 μmol/L) of Epigenetic Compound Library (TargetMol, MA, USA).

Techniques: Selection, In Vitro, Incubation, Western Blot, Analysis, Dot Blot, Immunohistochemistry, Staining, Immunofluorescence, Marker, Control, Comparison

Journal: Aging (Albany NY)

Article Title: Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients

doi: 10.18632/aging.205695

Figure Lengend Snippet: Methylation level of receptor accessory protein 4 ( REEP4 ) in lower-grade glioma (LGG) based on the TCGA database. ( A ) Methylation sites of REEP4 in LGG. ( B ) The expression level of REEP4 is negatively correlated with the total methylation level. ( C – H ) The expression level of REEP4 is negatively correlated with methylation sites cg13265914, cg16311504, cg07664173, cg20582089, cg19738333 and cg02498268. ( I ) The expression level of REEP4 is positively correlated with the methylation site cg02399048. ( J ) Effect of methylation site cg16311504 on the overall survival of patients with LGG. ( K ) Real-time quantitative polymerase chain reaction showing that the expression level of REEP4 was significantly inhibited by SAM. **** P < 0·0001.

Article Snippet: For the methylation assay, SHG-44 cells were treated with 100 uM of ademetionine disulfate tosylate (SAM) when their cell fusion approached 70% (Cat 97540-22-2, Topscience, China) for 10 h. The treated cells were harvested to identify the REEP4 RNA level.

Techniques: Methylation, Expressing, Real-time Polymerase Chain Reaction